Staining:Article Title: Epigenome profiling identifies H3K27me3 regulation of extracellular matrix composition in human corticogenesis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Maxpar Cell Staining Buffer Standard BioTools Cat # 201068 DPBS Corning Cat # 21-031-CV 16 % Formaldehyde (w/v), methanol-free Thermo Scientific Cat # 28906 Fetal Bovine Serum (FBS) Sigma Aldrich Cat # F7524 Maxpar Nuclear Antigen Staining Buffer Set Standard BioTools Cat # 201063 Maxpar Fix I Buffer Standard BioTools Cat # 201065 Maxpar 10X Barcode Perm Buffer Standard BioTools Cat # 201057 Cell-IDTM Intercalator-Iridium Standard BioTools Cat # 201192A Maxpar Water Standard BioTools Cat # 201069 Maxpar Cell Acquisition Solution Plus for CyTOF XT Standard BioTools Cat # 201244 EQTM Six Element Calibration Beads Standard BioTools Cat # 201245 Critical commercial assays MACS Neural Tissue Dissociation Kit P Miltenyi Biotec Inc. Cat # 130-092-628 RNeasy Mini Kit QIAGEN Cat # 74104 Neural Tissue Dissociation Kit (P) Miltenyi Biotec Inc. Cat # 130-092-628 Cell-ID 20-Plex Pd Barcoding Kit Standard BioTools Cat # 201060 Deposited data RNA-seq Florio et al.35 NCBI GEO: GSE65000 CUT&Tag Human tissue GW12-14 This paper Zenodo: https://doi.org/10.5281/ zenodo.13897222 CUT&Tag PRC2 inhibitor treated organoids W8 This paper Zenodo: https://doi.org/10.5281/ zenodo.13897222 Bulk RNA-seq PRC2 inhibitor treated organoids W8 This paper Zenodo: https://doi.org/10.5281/ zenodo.13897222 Epi-CyTOF This paper FlowRepository: FR-FCM-Z8DC Experimental models: Cell lines Human: CRTDi004-A iPSC Völkner et al.78 CRTDi004-A; RRID: CVCL_YR23 Human: HPSI0114i-kolf_2 iPSC Welcome Trust Sanger Institute, HipSci WTSIi018-B; RRID: CVCL_AE29 Oligonucleotides Primers, see Table S7 This paper N/A Software and algorithms Fiji/ImageJ Fiji/ImageJ https://imagej.nih.gov/ij/ Prism (8.4.3) GraphPad software N/A Affinity Photo + Designer (1.10.5.1342) Serif Ltd. N/A FACSDiva (8.0.2) BD Biosciences N/A FACSChorus BD Biosciences N/A CyTOF Software (v9.0.2) Standard BioTools N/A OMIQ Dotmatics N/A FastQC (v0.11.9) Babraham Bioinformatics https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ nf-core/cutandrun (v3.2.2) Cheshire et al.79 N/A Diffbind (v3.12.0) Stark et al.80 N/A DESeq2 (v1.42.1) Love et al.81 N/A deeptools (v3.5.1) Yu et al.82 N/A ChIPseeker (v1.38.0) Yu et al.82 and Bioconductor Core Team and Bioconductor Package Maintainer83 N/A ReactomePA (v1.46.0) Yu et al.44 and Moore et al.45 N/A (Continued on next page) e4 Neuron 113, 2927–2944.e1–e10, September 17, 2025 ..
Magnetic Cell Separation:Article Title: Epigenome profiling identifies H3K27me3 regulation of extracellular matrix composition in human corticogenesis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Maxpar Cell Staining Buffer Standard BioTools Cat # 201068 DPBS Corning Cat # 21-031-CV 16 % Formaldehyde (w/v), methanol-free Thermo Scientific Cat # 28906 Fetal Bovine Serum (FBS) Sigma Aldrich Cat # F7524 Maxpar Nuclear Antigen Staining Buffer Set Standard BioTools Cat # 201063 Maxpar Fix I Buffer Standard BioTools Cat # 201065 Maxpar 10X Barcode Perm Buffer Standard BioTools Cat # 201057 Cell-IDTM Intercalator-Iridium Standard BioTools Cat # 201192A Maxpar Water Standard BioTools Cat # 201069 Maxpar Cell Acquisition Solution Plus for CyTOF XT Standard BioTools Cat # 201244 EQTM Six Element Calibration Beads Standard BioTools Cat # 201245 Critical commercial assays MACS Neural Tissue Dissociation Kit P Miltenyi Biotec Inc. Cat # 130-092-628 RNeasy Mini Kit QIAGEN Cat # 74104 Neural Tissue Dissociation Kit (P) Miltenyi Biotec Inc. Cat # 130-092-628 Cell-ID 20-Plex Pd Barcoding Kit Standard BioTools Cat # 201060 Deposited data RNA-seq Florio et al.35 NCBI GEO: GSE65000 CUT&Tag Human tissue GW12-14 This paper Zenodo: https://doi.org/10.5281/ zenodo.13897222 CUT&Tag PRC2 inhibitor treated organoids W8 This paper Zenodo: https://doi.org/10.5281/ zenodo.13897222 Bulk RNA-seq PRC2 inhibitor treated organoids W8 This paper Zenodo: https://doi.org/10.5281/ zenodo.13897222 Epi-CyTOF This paper FlowRepository: FR-FCM-Z8DC Experimental models: Cell lines Human: CRTDi004-A iPSC Völkner et al.78 CRTDi004-A; RRID: CVCL_YR23 Human: HPSI0114i-kolf_2 iPSC Welcome Trust Sanger Institute, HipSci WTSIi018-B; RRID: CVCL_AE29 Oligonucleotides Primers, see Table S7 This paper N/A Software and algorithms Fiji/ImageJ Fiji/ImageJ https://imagej.nih.gov/ij/ Prism (8.4.3) GraphPad software N/A Affinity Photo + Designer (1.10.5.1342) Serif Ltd. N/A FACSDiva (8.0.2) BD Biosciences N/A FACSChorus BD Biosciences N/A CyTOF Software (v9.0.2) Standard BioTools N/A OMIQ Dotmatics N/A FastQC (v0.11.9) Babraham Bioinformatics https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ nf-core/cutandrun (v3.2.2) Cheshire et al.79 N/A Diffbind (v3.12.0) Stark et al.80 N/A DESeq2 (v1.42.1) Love et al.81 N/A deeptools (v3.5.1) Yu et al.82 N/A ChIPseeker (v1.38.0) Yu et al.82 and Bioconductor Core Team and Bioconductor Package Maintainer83 N/A ReactomePA (v1.46.0) Yu et al.44 and Moore et al.45 N/A (Continued on next page) e4 Neuron 113, 2927–2944.e1–e10, September 17, 2025 ..
RNA Sequencing:Article Title: Epigenome profiling identifies H3K27me3 regulation of extracellular matrix composition in human corticogenesis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Maxpar Cell Staining Buffer Standard BioTools Cat # 201068 DPBS Corning Cat # 21-031-CV 16 % Formaldehyde (w/v), methanol-free Thermo Scientific Cat # 28906 Fetal Bovine Serum (FBS) Sigma Aldrich Cat # F7524 Maxpar Nuclear Antigen Staining Buffer Set Standard BioTools Cat # 201063 Maxpar Fix I Buffer Standard BioTools Cat # 201065 Maxpar 10X Barcode Perm Buffer Standard BioTools Cat # 201057 Cell-IDTM Intercalator-Iridium Standard BioTools Cat # 201192A Maxpar Water Standard BioTools Cat # 201069 Maxpar Cell Acquisition Solution Plus for CyTOF XT Standard BioTools Cat # 201244 EQTM Six Element Calibration Beads Standard BioTools Cat # 201245 Critical commercial assays MACS Neural Tissue Dissociation Kit P Miltenyi Biotec Inc. Cat # 130-092-628 RNeasy Mini Kit QIAGEN Cat # 74104 Neural Tissue Dissociation Kit (P) Miltenyi Biotec Inc. Cat # 130-092-628 Cell-ID 20-Plex Pd Barcoding Kit Standard BioTools Cat # 201060 Deposited data RNA-seq Florio et al.35 NCBI GEO: GSE65000 CUT&Tag Human tissue GW12-14 This paper Zenodo: https://doi.org/10.5281/ zenodo.13897222 CUT&Tag PRC2 inhibitor treated organoids W8 This paper Zenodo: https://doi.org/10.5281/ zenodo.13897222 Bulk RNA-seq PRC2 inhibitor treated organoids W8 This paper Zenodo: https://doi.org/10.5281/ zenodo.13897222 Epi-CyTOF This paper FlowRepository: FR-FCM-Z8DC Experimental models: Cell lines Human: CRTDi004-A iPSC Völkner et al.78 CRTDi004-A; RRID: CVCL_YR23 Human: HPSI0114i-kolf_2 iPSC Welcome Trust Sanger Institute, HipSci WTSIi018-B; RRID: CVCL_AE29 Oligonucleotides Primers, see Table S7 This paper N/A Software and algorithms Fiji/ImageJ Fiji/ImageJ https://imagej.nih.gov/ij/ Prism (8.4.3) GraphPad software N/A Affinity Photo + Designer (1.10.5.1342) Serif Ltd. N/A FACSDiva (8.0.2) BD Biosciences N/A FACSChorus BD Biosciences N/A CyTOF Software (v9.0.2) Standard BioTools N/A OMIQ Dotmatics N/A FastQC (v0.11.9) Babraham Bioinformatics https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ nf-core/cutandrun (v3.2.2) Cheshire et al.79 N/A Diffbind (v3.12.0) Stark et al.80 N/A DESeq2 (v1.42.1) Love et al.81 N/A deeptools (v3.5.1) Yu et al.82 N/A ChIPseeker (v1.38.0) Yu et al.82 and Bioconductor Core Team and Bioconductor Package Maintainer83 N/A ReactomePA (v1.46.0) Yu et al.44 and Moore et al.45 N/A (Continued on next page) e4 Neuron 113, 2927–2944.e1–e10, September 17, 2025 ..
Software:Article Title: Epigenome profiling identifies H3K27me3 regulation of extracellular matrix composition in human corticogenesis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Maxpar Cell Staining Buffer Standard BioTools Cat # 201068 DPBS Corning Cat # 21-031-CV 16 % Formaldehyde (w/v), methanol-free Thermo Scientific Cat # 28906 Fetal Bovine Serum (FBS) Sigma Aldrich Cat # F7524 Maxpar Nuclear Antigen Staining Buffer Set Standard BioTools Cat # 201063 Maxpar Fix I Buffer Standard BioTools Cat # 201065 Maxpar 10X Barcode Perm Buffer Standard BioTools Cat # 201057 Cell-IDTM Intercalator-Iridium Standard BioTools Cat # 201192A Maxpar Water Standard BioTools Cat # 201069 Maxpar Cell Acquisition Solution Plus for CyTOF XT Standard BioTools Cat # 201244 EQTM Six Element Calibration Beads Standard BioTools Cat # 201245 Critical commercial assays MACS Neural Tissue Dissociation Kit P Miltenyi Biotec Inc. Cat # 130-092-628 RNeasy Mini Kit QIAGEN Cat # 74104 Neural Tissue Dissociation Kit (P) Miltenyi Biotec Inc. Cat # 130-092-628 Cell-ID 20-Plex Pd Barcoding Kit Standard BioTools Cat # 201060 Deposited data RNA-seq Florio et al.35 NCBI GEO: GSE65000 CUT&Tag Human tissue GW12-14 This paper Zenodo: https://doi.org/10.5281/ zenodo.13897222 CUT&Tag PRC2 inhibitor treated organoids W8 This paper Zenodo: https://doi.org/10.5281/ zenodo.13897222 Bulk RNA-seq PRC2 inhibitor treated organoids W8 This paper Zenodo: https://doi.org/10.5281/ zenodo.13897222 Epi-CyTOF This paper FlowRepository: FR-FCM-Z8DC Experimental models: Cell lines Human: CRTDi004-A iPSC Völkner et al.78 CRTDi004-A; RRID: CVCL_YR23 Human: HPSI0114i-kolf_2 iPSC Welcome Trust Sanger Institute, HipSci WTSIi018-B; RRID: CVCL_AE29 Oligonucleotides Primers, see Table S7 This paper N/A Software and algorithms Fiji/ImageJ Fiji/ImageJ https://imagej.nih.gov/ij/ Prism (8.4.3) GraphPad software N/A Affinity Photo + Designer (1.10.5.1342) Serif Ltd. N/A FACSDiva (8.0.2) BD Biosciences N/A FACSChorus BD Biosciences N/A CyTOF Software (v9.0.2) Standard BioTools N/A OMIQ Dotmatics N/A FastQC (v0.11.9) Babraham Bioinformatics https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ nf-core/cutandrun (v3.2.2) Cheshire et al.79 N/A Diffbind (v3.12.0) Stark et al.80 N/A DESeq2 (v1.42.1) Love et al.81 N/A deeptools (v3.5.1) Yu et al.82 N/A ChIPseeker (v1.38.0) Yu et al.82 and Bioconductor Core Team and Bioconductor Package Maintainer83 N/A ReactomePA (v1.46.0) Yu et al.44 and Moore et al.45 N/A (Continued on next page) e4 Neuron 113, 2927–2944.e1–e10, September 17, 2025 ..
Concentration Assay:Article Title: Epigenome profiling identifies H3K27me3 regulation of extracellular matrix composition in human corticogenesis.
Article Snippet: The cell pellet was washed with 2 ml Maxpar water (Standard BioTools, 201069), filtered through a 35 μm cell strainer (Falcon, 352235) and counted with the MACSQuant Analyzer (Miltenyi Biotec). .. For cell acquisition, the cell concentration was adjusted to 6 x 105 cells per ml with Maxpar cell acquisition solution plus (CASplus, Standard BioTools, 201244). .. An instrument calibration and tuning were performed using EQ six element calibration beads (Standard BioTools, 201245) before sample analysis.
other:
|
fluidigm is a verified supplier 
fluidigm manufactures this product 